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| Registros recuperados: 237 | |
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| Sousa,Cristina Paiva de. |
| Food-borne illness is a major international problem and an important cause of reduced economic growth. The contamination of the food supply with the pathogens and its persistence, growth, multiplication and/or toxin production has emerged as an important public health concern. Most of these problems could be controlled with the efforts on the part of the food handlers, whether in a processing plant, a restaurant, and others. In contrast with most chemical hazardous compounds, the concentration of food pathogens changes during the processing, storage, and meal preparation, making it difficult to estimate the number of the microorganisms or the concentration of their toxins at the time of ingestion by the consumer. This review shows main microorganisms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food-borne diseases; Staphylococcus spp.; Escherichia coli; Salmonella spp.; Food handlers. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400020 |
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| Hamadi,Fatima; Latrache,Hassan; Zahir,Hafida; Elghmari,Abderrahmene; Timinouni,Mohamed; Ellouali,Mostapha. |
| Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Surface functional groups; Hydrophobicity; Electrophoretic mobility. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100003 |
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| Fernandes,C.I.; Llimona,F.; Godoy,L.C.; Negri,E.M.; Pontieri,V.; Moretti,A.I.; Fernandes,T.R.; Soriano,F.G.; Velasco,I.T.; Souza,H.P.. |
| Shock and resuscitation render patients more susceptible to acute lung injury due to an exacerbated immune response to subsequent inflammatory stimuli. To study the role of innate immunity in this situation, we investigated acute lung injury in an experimental model of ischemia-reperfusion (I-R) followed by an early challenge with live bacteria. Conscious rats (N = 8 in each group) were submitted to controlled hemorrhage and resuscitated with isotonic saline (SS, 0.9% NaCl) or hypertonic saline (HS, 7.5% NaCl) solution, followed by intratracheal or intraperitoneal inoculation of Escherichia coli. After infection, toll-like receptor (TLR) 2 and 4 mRNA expression was monitored by RT-PCR in infected tissues. Plasma levels of tumor necrosis factor α and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toll-like receptors; Immune system; Pneumonia; Escherichia coli; Neutrophils. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000004 |
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| Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Nunes,Maurício Pinto; Santana,Elsa Helena Walter de; Nero,Luís Augusto; Souza,Juliana Aparecida de. |
| Readycult - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult - LMX. The Readycult - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Readycult - LMX; X-GAL; MUG; Milk; Escherichia coli; Total coliforms. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010 |
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| Boratto,Adriano José; Lopes,Darci Clementino; Oliveira,Rita Flávia Miranda de; Albino,Luiz Fernando Teixeira; Sá,Luciano Moraes; Oliveira,Gisele Andrade de. |
| Para avaliar o efeito do uso de antibiótico, de probiótico e de homeopatia em frangos de corte, inoculados ou não com Escherichia coli, foram distribuídos 672 frangos machos, Avian Farm, em delineamento inteiramente casualizado em esquema fatorial com oito tratamentos (quatro aditivos x com ou sem inoculação) e seis repetições, tendo 14, 12 e 7 aves por unidade experimental, respectivamente, de acordo com os períodos 1 a 11, 12 a 21 e 22 a 42 dias de idade. As aves foram alojadas em salas climatizadas em ambiente de conforto, de acordo com o manual da linhagem. Os tratamentos foram constituídos de: controle negativo (sem aditivo), antibiótico (virginiamicina + nitrovin), probiótico (meio de cultura de Lactobacilus acidophillus, Enterococcus faecium e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ambiente; Escherichia coli; Frango de corte; Promotor de crescimento. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600014 |
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| Ribeiro,Andréa Machado Leal; Rudnik,Liliane; Canal,Cláudio Wageck; Kratz,Lilian Ribeiro; Farias,Carolina. |
| Foi avaliado o efeito das gemas de ovos de aves hiperimunizadas contra Escherichia coli (E. coli) patogênica para suínos sobre a imunidade passiva de leitões recém-nascidos em uma unidade produtora de leitões. Foram avaliados a densidade ótica do ELISA (DO) para anticorpos contra E. coli, o peso corporal e a ocorrência de diarréia diária em 137 leitões recém-nascidos oriundos de 25 fêmeas primíparas, não vacinadas contra E. coli. As porcas foram consideradas blocos. De cada fêmea, foram separados seis leitões recém-nascidos de ambos os sexos, excluindo-se os mais leves e os mais pesados, divididos em três tratamentos e duas repetições. Os seguintes tratamentos foram fornecidos via oral: T1: 2 mL de PBS (tratamento controle) em duas doses, a primeira ao... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarréia; Escherichia coli; Gema; Imunoglobulina Y; Imunidade passiva; Leitões. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982005000400019 |
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| Maia,Sandra Ribeiro; Ferreira,Ana Cristina; Abreu,Luiz Ronaldo de. |
| Considerando o envolvimento de queijos como veículo de microrganismos patogênicos, foi avaliada a eficiência do extrato alcoólico de cúrcuma adicionado à ricota, na redução de Escherichia coli e Enterobacter aerogenes. Foram fabricados três lotes de ricota cremosa e inoculados com 104 UFC/mL de Escherichia coli (ATCC 25922) e 105 UFC/mL de Enterobacter aerogenes (ATCC 13048). Às ricotas, foram adicionados 0,4% de NaCl e extrato alcoólico de Curcuma longa L., em concentrações que variaram de 0,0% a 2,0%. As ricotas foram avaliadas físico-química e microbiologicamente em 0, 1, 7, 14 e 21 dias de armazenamento refrigerado. O percentual de umidade das ricotas foi, em média, de 73%. O pH médio observado foi de 5,4 e o percentual de gordura de 3%. Pelos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcuma longa; Escherichia coli; Enterobacter aerogenes; Ricota. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000200016 |
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| Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan. |
| ABSTRACT: This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451 |
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| Rigobelo,E.C.; Gamez,H.J.; Marin,J.M.; Macedo,C.; Ambrosin,J.A.; Ávila,F.A.. |
| One hundred seventy-three Escherichia coli strains isolated from calves from northwestern São Paulo State, having diarrhea were examined for the production of thermolabile (LT) and thermostable (ST) enterotoxins and for the presence of virulence factors associated with bovine colibacillosis. Eighty-five (49.1%) of the E.coli strains produced toxins; 53 isolates were detected as producing STa toxin, and 9 also produced LT toxin. By PCR, 23 isolates were shown to harbor only the LT-II gene. Nine (5.2%) isolates harbored Shiga toxin genes: four carried the stx2 gene, four the stx1 gene and one carried both. Three of the isolates showing stx1 also carried the eae gene. Among the E. coli isolates examined for susceptibility to 10 antimicrobial agents,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calf; Diarrhea; Escherichia coli; Virulence factor; Antimicrobial agent. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300003 |
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| Carneiro,V.C.; Lessa,D.A.B.; Guttmann,P.M.; Magalhaes,H.; Aquino,M.H.C.; Cunha,L.E.R.; Arais,L.R.; Cerqueira,A.M.F.. |
| ABSTRACT Respiratory diseases are common in young horses but little is known about such infections in mule foals. This study aimed to characterize Escherichia coli and Klebsiella sp. isolated from tracheal wash (TW) and fecal samples (FS) of mule foals, with or without cytological evidence of respiratory disease. Strains were analyzed against 13 antimicrobials, for presence of Extended spectrum beta-lactamase (ESBL), and virulence genes. Phylogrouping and Randomic (RAPD)-PCR profiles were used to evaluate their genetic relatedness. E. coli strains from TW and FS showed greatest resistance to tetracycline, while Klebsiella strains were mainly resistant to ampicillin; multidrug resistance and ESBL production were also detected. The blaCTX gene prevailed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Klebsiella; Respiratory disease; Virulence; Antimicrobial resistance. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501073 |
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| Registros recuperados: 237 | |
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